Nanocomposite of cosubstituted carbonated hydroxyapatite fabricated inside Poly(sodium hyaluronate-acrylamide) hydrogel template prepared by gamma radiation for osteoblast cell regeneration

Carbonated hydroxyapatite (CCaHAp) closely mimics the chemical composition of apatite found in human bone, compared to (HA). The present study aimed prepare CCaHAp nanocomposite using a copolymer sodium hyaluronate-polyacrylamide (poly(Hya-AAm)) hydrogel as HA template. Here, gamma irradiation technique was utilized for preparing poly(Hya-AAm) hydrogel. had dual function template and carbon precursor. synthesized situ by impregnating swollen discs solution phosphoric acid calcium nitrate. impregnation cycle repeated one, two, three, four times fabricate samples with Ca/P ratios 1.67, 1.89, 1.48, 1.37 content 8.6, 12.25, 12.24, 10.07%, respectively, minor cosubstitution Na Mg atoms. Moreover, this analyzed effectiveness HA, which used control vitro osteoblast cell regeneration. Four were seeded MC3T3-E1 cells. On day 1, increased attachment observed that discs. Furthermore, control, differentiation regeneration significantly on 21, especially sample ratio 1.89. This use nanobiocompatible material potentially improved osteogenic ability HA.